The data show that mitochondrial glycolate oxidation contributes to photorespiration in higher plants. Furthermore, photorespiration is reduced in these mutant lines compared with the wild type, as revealed by determination of the post-illumination CO2 burst and the glycine/serine ratio under photorespiratory growth conditions. 25 Pages 593-599 Figure 1: The E.coli glycolate catabolic pathway (red) superimposed on plant photorespiration (black). Author(s): Rashad Kebeish 1, Markus Niessen 1, Krishnaveni Thiruveedhi 1, Rafijul Bari 1, Heinz-Josef Hirsch 1, Ruben Rosenkranz 1, Norma Stbler 1. T-DNA insertion lines for AtGDH show a drastic reduction in mitochondrial GDH activity and CO2 release from glycolate. Kebeish, Markus Niessen, Krishnaveni Thiruveedhi, Rafijul Bari, Heinz-Josef Hirsch, Ruben Rosenkranz, Norma Stbler, Barbara Schnfeld, Fritz Kreuzaler and Christoph Peterhnsel. This effect could be enhanced by adding co-factors for aminotransferases, but is inhibited by the addition of glycine. Thupakula Krishnaveni Nemali Sudha 9490893783 1-461 new indhira nagar Sangitham Sugunamma 9000949856 1-463-B indira nagar VEERAMALLI KRISHNAIAH 8977511858 461-46 INDIRA NAGAR Tupili Chengamma 1/461-223-B indira nagar Shaik Basha 9346794678 1-2-134 indira nagar Shaik Jilanibasha 8985832024 V PADMA 1-461-384-A INDIRA NAGAR KOMMI RATHNAMMA. Kebeish, Markus Niessen, Krishnaveni Thiruveedhi, Rafijul Bari, Heinz-Josef Hirsch, Ruben Rosenkranz, Norma Stäbler, Barbara Schönfeld, Fritz Kreuzaler and Christoph Peterhänsel. Labelling experiments indicated that glycolate oxidation in mitochondrial extracts is coupled to CO2 release. Both RNA abundance and mitochondrial GDH activity were up-regulated under photorespiratory growth conditions. In this study, the contribution of AtGDH to photorespiration was characterized. Previous analyses revealed the existence of a GDH in the mitochondria of Arabidopsis thaliana (AtGDH). Land plants and charophycean green algae oxidize glycolate in the peroxisome using oxygen as a co-factor, whereas chlorophycean green algae use a mitochondrial glycolate dehydrogenase (GDH) with organic co-factors. Chloroplastic Photorespiratory Bypass Increases Photosynthesis and Biomass Production in Arabidopsis Thaliana. The oxidation of glycolate to glyoxylate is an important reaction step in photorespiration. Kebeish, Rashad, Markus Niessen, Krishnaveni Thiruveedhi, Rafijul Bari, Heinz-Josef Hirsch, Ruben Rosenkranz, Norma Stäbler, Barbara Schönfeld, Fritz Kreuzaler, and Christoph Peterhänsel.
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